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pde4 activity assay kit  (Abcam)

 
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    Abcam pde4 activity assay kit
    PDE4D exacerbates Ang II-induced vasocontraction by affecting SMC contraction, consequently contributing to hypertension. The mechanism by which PDE4D aggravates SMC contraction likely involves the PKA-AMPK-MYPT1-MLC signaling pathway. <t>PDE4</t> inhibitor, rolipram, showed a therapeutic effect on Ang II-induced hypertension in mice primarily through PDE4D in SMCs. The image was created with BioRender.com, and acquired the permission for use and proper accreditation.
    Pde4 Activity Assay Kit, supplied by Abcam, used in various techniques. Bioz Stars score: 92/100, based on 63 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/pde4 activity assay kit/product/Abcam
    Average 92 stars, based on 63 article reviews
    pde4 activity assay kit - by Bioz Stars, 2026-03
    92/100 stars

    Images

    1) Product Images from "Phosphodiesterase 4D promotes angiotensin II-induced hypertension in mice via smooth muscle cell contraction"

    Article Title: Phosphodiesterase 4D promotes angiotensin II-induced hypertension in mice via smooth muscle cell contraction

    Journal: Communications Biology

    doi: 10.1038/s42003-022-03029-0

    PDE4D exacerbates Ang II-induced vasocontraction by affecting SMC contraction, consequently contributing to hypertension. The mechanism by which PDE4D aggravates SMC contraction likely involves the PKA-AMPK-MYPT1-MLC signaling pathway. PDE4 inhibitor, rolipram, showed a therapeutic effect on Ang II-induced hypertension in mice primarily through PDE4D in SMCs. The image was created with BioRender.com, and acquired the permission for use and proper accreditation.
    Figure Legend Snippet: PDE4D exacerbates Ang II-induced vasocontraction by affecting SMC contraction, consequently contributing to hypertension. The mechanism by which PDE4D aggravates SMC contraction likely involves the PKA-AMPK-MYPT1-MLC signaling pathway. PDE4 inhibitor, rolipram, showed a therapeutic effect on Ang II-induced hypertension in mice primarily through PDE4D in SMCs. The image was created with BioRender.com, and acquired the permission for use and proper accreditation.

    Techniques Used:



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    pde4 activity assay kit  (Abcam)
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    PDE4D exacerbates Ang II-induced vasocontraction by affecting SMC contraction, consequently contributing to hypertension. The mechanism by which PDE4D aggravates SMC contraction likely involves the PKA-AMPK-MYPT1-MLC signaling pathway. <t>PDE4</t> inhibitor, rolipram, showed a therapeutic effect on Ang II-induced hypertension in mice primarily through PDE4D in SMCs. The image was created with BioRender.com, and acquired the permission for use and proper accreditation.
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    pde4 activity assay kit  (Promega)
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    A–C. The chemical structure of Forsythin extracted from Forsythia suspensa seeds. Forsythin is an o-linked β-D-glucopyranosylated lignin that can be hydrolyzed (Red line). D. Predicted docking site of Forsythin with <t>PDE4.</t> E. Predicted residue electrostatic and van der Waals interactions between PDE4 amino acids and Forsythin. Using ZINCPharmer , a pharmacophore model was generated (upper left) and used to screen the lead compounds from an 18.3 million purchasable compound library.
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    Image Search Results


    PDE4D exacerbates Ang II-induced vasocontraction by affecting SMC contraction, consequently contributing to hypertension. The mechanism by which PDE4D aggravates SMC contraction likely involves the PKA-AMPK-MYPT1-MLC signaling pathway. PDE4 inhibitor, rolipram, showed a therapeutic effect on Ang II-induced hypertension in mice primarily through PDE4D in SMCs. The image was created with BioRender.com, and acquired the permission for use and proper accreditation.

    Journal: Communications Biology

    Article Title: Phosphodiesterase 4D promotes angiotensin II-induced hypertension in mice via smooth muscle cell contraction

    doi: 10.1038/s42003-022-03029-0

    Figure Lengend Snippet: PDE4D exacerbates Ang II-induced vasocontraction by affecting SMC contraction, consequently contributing to hypertension. The mechanism by which PDE4D aggravates SMC contraction likely involves the PKA-AMPK-MYPT1-MLC signaling pathway. PDE4 inhibitor, rolipram, showed a therapeutic effect on Ang II-induced hypertension in mice primarily through PDE4D in SMCs. The image was created with BioRender.com, and acquired the permission for use and proper accreditation.

    Article Snippet: PDE4 activity in RASMCs was detected via a PDE4 activity assay kit (Abcam, Cat#: ab139460) according to the manufacturer’s instructions.

    Techniques:

    A–C. The chemical structure of Forsythin extracted from Forsythia suspensa seeds. Forsythin is an o-linked β-D-glucopyranosylated lignin that can be hydrolyzed (Red line). D. Predicted docking site of Forsythin with PDE4. E. Predicted residue electrostatic and van der Waals interactions between PDE4 amino acids and Forsythin. Using ZINCPharmer , a pharmacophore model was generated (upper left) and used to screen the lead compounds from an 18.3 million purchasable compound library.

    Journal: PLoS ONE

    Article Title: Novel PDE4 Inhibitors Derived from Chinese Medicine Forsythia

    doi: 10.1371/journal.pone.0115937

    Figure Lengend Snippet: A–C. The chemical structure of Forsythin extracted from Forsythia suspensa seeds. Forsythin is an o-linked β-D-glucopyranosylated lignin that can be hydrolyzed (Red line). D. Predicted docking site of Forsythin with PDE4. E. Predicted residue electrostatic and van der Waals interactions between PDE4 amino acids and Forsythin. Using ZINCPharmer , a pharmacophore model was generated (upper left) and used to screen the lead compounds from an 18.3 million purchasable compound library.

    Article Snippet: PDE4 activity assay kit was from Promega.

    Techniques: Residue, Generated, Drug discovery

    For the PDE activity assay, all test compounds were diluted in DMSO with final concentrations in each assay of 100, 10, 1, 0.1, 0.01, 0.001, 0.0001 µM. For the PDE4 activity assay, 10 mU of purified PDE4 (Millipore) was used per reaction. Compounds 6, 7, 9 and 13 (blue underline) were further tested in PDE3, 5, 7, 10 activity assays in which 25 mU of purified enzyme was used per reaction. Summary of compound IC 50 in lower right corner.

    Journal: PLoS ONE

    Article Title: Novel PDE4 Inhibitors Derived from Chinese Medicine Forsythia

    doi: 10.1371/journal.pone.0115937

    Figure Lengend Snippet: For the PDE activity assay, all test compounds were diluted in DMSO with final concentrations in each assay of 100, 10, 1, 0.1, 0.01, 0.001, 0.0001 µM. For the PDE4 activity assay, 10 mU of purified PDE4 (Millipore) was used per reaction. Compounds 6, 7, 9 and 13 (blue underline) were further tested in PDE3, 5, 7, 10 activity assays in which 25 mU of purified enzyme was used per reaction. Summary of compound IC 50 in lower right corner.

    Article Snippet: PDE4 activity assay kit was from Promega.

    Techniques: Activity Assay, Purification